Printable Datasheet

Recombinant CEACAM1 (CEA Cell Adhesion Molecule 1) Antibody [r29H2]

In Stock
Catalog Number Formulation Size Price
634-MSM36-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
634-MSM36-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
634-MSM36-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CEACAM1 Antibody in SDS-PAGE

CEACAM1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CEACAM1 Recombinant Mouse Monoclonal Antibody (r29H2). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD66a antigen, also known as biliary glycoprotein (BGP), is a member of the carcinoembryonic antigen (CEA) family and of the immunoglobulin superfamily. Products of four of the seven functional CEA family genes (CD66a, CD66b, CD66c and CD66d) are known to be expressed by hematopoietic cells. CD66a antigen is expressed in epithelia, in certain endothelia and in cells of the myeloid lineage.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Apoptosis, Immunology, Angiogenesis, Hematopoietic Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CEACAM1
Positive Control
Human colon, liver, endometrium or hepatic carcinoma tissues.
Immunogen
Prokaryotic recombinant protein corresponding to a 170 amino acid truncate (266 to 436aa) of the CD66a molecule.
Alternate Names
Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, Antigen CD66; BGP-1; BGPI; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); CD66a; CEACAM1; meconium antigen 100

Database Links

Entrez Gene ID
634
SwissProt
P13688

Additional Information

Clone
r29H2
Chromosome Location
19q13.2
Mol. Weight of Antigen
90-180kDa
Expression System
CHO Cells

Related Antibodies

View All CEACAM1 Antibodies

Functions

  • Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity). Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis (PubMed:18424730, PubMed:23696226, PubMed:25363763). Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils (PubMed:18424730, PubMed:23696226). Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70 (PubMed:18424730). Also inhibits T cell proliferation and cytokine production through inhibition of JNK cascade and plays a crucial role in regulating autoimmunity and anti-tumor immunity by inhibiting T cell through its interaction with HAVCR2 (PubMed:25363763). Upon natural killer (NK) cells activation, inhibit KLRK1-mediated cytolysis of CEACAM1-bearing tumor cells by trans-homophilic interactions with CEACAM1 on the target cell and lead to cis-interaction between CEACAM1 and KLRK1, allowing PTPN6 recruitment and then VAV1 dephosphorylation (PubMed:23696226). Upon neutrophils activation negatively regulates IL1B production by recruiting PTPN6 to a SYK-TLR4-CEACAM1 complex, that dephosphorylates SYK, reducing the production of reactive oxygen species (ROS) and lysosome disruption, which in turn, reduces the activity of the inflammasome. Down-regulates neutrophil production by acting as a coinhibitory receptor for CSF3R by down-regulating the CSF3R-STAT3 pathway through recruitment of PTPN6 that dephosphorylates CSF3R (By similarity). Also regulates insulin action by promoting INS clearance and regulating lipogenesis in liver through regulating insulin signaling (By similarity). Upon INS stimulation, undergoes phosphorylation by INSR leading to INS clearance by increasing receptor-mediated insulin endocytosis. This inernalization promotes interaction with FASN leading to receptor-mediated insulin degradation and to reduction of FASN activity leading to negative regulation of fatty acid synthesis. INSR-mediated phosphorylation also provokes a down-regulation of cell proliferation through SHC1 interaction resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways (By similarity). Functions as activator in angiogenesis by promoting blood vessel remodeling through endothelial cell differentiation and migration and in arteriogenesis by increasing the number of collateral arteries and collateral vessel calibers after ischemia. Also regulates vascular permeability through the VEGFR2 signaling pathway resulting in control of nitric oxide production (By similarity). Down-regulates cell growth in response to EGF through its interaction with SHC1 that mediates interaction with EGFR resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 pathway (By similarity). Negatively regulates platelet aggregation by decreasing platelet adhesion on type I collagen through the GPVI-FcRgamma complex (By similarity). Inhibits cell migration and cell scattering through interaction with FLNA; interfers with the interaction of FLNA with RALA (PubMed:16291724). Mediates bile acid transport activity in a phosphorylation dependent manner (By similarity). Negatively regulates osteoclastogenesis (By similarity).
  • Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity). Promotes populations of T cells regulating IgA production and secretion associated with control of the commensal microbiota and resistance to enteropathogens (By similarity).

Key References

  • Miura M, et al. Exp Cell Res 1996;226:126-132.
  • Bamberger, A M et al. “The adhesion molecule CEACAM1 (CD66a, C-CAM, BGP) is specifically expressed by the extravillous intermediate trophoblast.” The American journal of pathology vol. 156,4 (2000): 1165-70. doi:10.1016/S0002-9440(10)64985-1
  • Morales, V M et al. “Regulation of human intestinal intraepithelial lymphocyte cytolytic function by biliary glycoprotein (CD66a).” Journal of immunology (Baltimore, Md. : 1950) vol. 163,3 (1999): 1363-70.
  • Huang, J et al. “Expression of biliary glycoprotein (CD66a) in normal and malignant breast epithelial cells.” Anticancer research vol. 18,5A (1998): 3203-12.
  • Riethdorf, L et al. “Differential expression of CD66a (BGP), a cell adhesion molecule of the carcinoembryonic antigen family, in benign, premalignant, and malignant lesions of the human mammary gland.” The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society vol. 45,7 (1997): 957-63. doi:10.1177/002215549704500705
  • Prall, F et al. “CD66a (BGP), an adhesion molecule of the carcinoembryonic antigen family, is expressed in epithelium, endothelium, and myeloid cells in a wide range of normal human tissues.” The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society vol. 44,1 (1996): 35-41. doi:10.1177/44.1.8543780
  • Hinoda, Y et al. “Molecular cloning of a cDNA coding biliary glycoprotein I: primary structure of a glycoprotein immunologically crossreactive with carcinoembryonic antigen.” Proceedings of the National Academy of Sciences of the United States of America vol. 85,18 (1988): 6959-63. doi:10.1073/pnas.85.18.6959
  • Li, Ning et al. “Carcinoembryonic antigen-related cell adhesion molecule 1 is expressed and as a function histotype in ovarian tumors.” Annals of diagnostic pathology vol. 20 (2016): 7-12. doi:10.1016/j.anndiagpath.2015.10.012
  • Wang, Ning et al. “Carcinoembryonic antigen cell adhesion molecule 1 inhibits the antitumor effect of neutrophils in tongue squamous cell carcinoma.” Cancer science vol. 110,2 (2019): 519-529. doi:10.1111/cas.13909

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant CEACAM1 (CEA Cell Adhesion Molecule 1) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales