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CDw75 (B-Cell Marker) Antibody [LN-1]

Additional information

Catalog Number: 6480-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6480-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6480-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6480-MSM1-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CDw75 (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (LN-1).

CDw75 (B-Cell Marker) Antibody - Image 2

Surface staining of human lymphocytes with CDw75 Mouse Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.

CDw75 (B-Cell Marker) Antibody - Image 3

Immunofluorescence Analysis of MeOH-fixed HeLa cells labeled with CDw75 Mouse Monoclonal Antibody (LN-1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Clone
LN-1
Isotype
IgM
Light Chain
Kappa
Cellular Localization
Golgi apparatus, Golgi stack membrane, Secreted
Gene Name
ST6GAL1
Positive Control
HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.
Immunogen
Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Key References
  • Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.
  • Marder RJ et. al. Lab Invest 52: 497-504, 1985.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6480
SwissProt
P15907
Chromosome Location
3q27.3
Mol. Weight of Antigen
46kDa
Alternate Names
Beta-galactoside alpha-2,6-sialyltransferase 1, B-cell antigen CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6Gal I, Sialyltransferase 1, 6-sialyltransferase 1 (6-ST1), Alpha 2,6 ST1, Beta galactoside alpha 2,6 sialyltransferase 1, CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, SIAT1, ST6 beta galactosamide alpha 2,6 sialyltranferase 1, ST6Gal I, ST6N

Summary

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

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