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CDw75 (B-Cell Marker) Antibody [LN-1]

In Stock
Catalog Number Formulation Size Price
6480-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6480-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6480-MSM1-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (LN-1).

Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (LN-1).

Surface staining of human lymphocytes with CDw75 Mouse Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.

Surface staining of human lymphocytes with CDw75 Mouse Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.

Immunofluorescence Analysis of MeOH-fixed HeLa cells labeled with CDw75 Mouse Monoclonal Antibody (LN-1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Immunofluorescence Analysis of MeOH-fixed HeLa cells labeled with CDw75 Mouse Monoclonal Antibody (LN-1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Infectious Disease
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Golgi apparatus, Golgi stack membrane, Secreted
Gene Name
ST6GAL1
Positive Control
HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.
Immunogen
Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
Alternate Names
Beta-galactoside alpha-2,6-sialyltransferase 1, B-cell antigen CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6Gal I, Sialyltransferase 1, 6-sialyltransferase 1 (6-ST1), Alpha 2,6 ST1, Beta galactoside alpha 2,6 sialyltransferase 1, CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, SIAT1, ST6 beta galactosamide alpha 2,6 sialyltranferase 1, ST6Gal I, ST6N

Database Links

Entrez Gene ID
6480
SwissProt
P15907

Additional Information

Clone
LN-1
Chromosome Location
3q27.3
Mol. Weight of Antigen
46kDa

Related Antibodies

View All ST6GAL1 Antibodies

Functions

  • Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.

Key References

  • Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.
  • Marder RJ et. al. Lab Invest 52: 497-504, 1985.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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