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CD50 / ICAM3 Antibody [SPM505]

Additional information

Catalog Number: 3385-MSM1X
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3385-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3385-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3385-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD50 / ICAM3 Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (SPM505)

CD50 Antibody in SDS-PAGE

CD50 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD50 Mouse Monoclonal Antibody (SPM505). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
SPM505
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Membrane
Gene Name
ICAM3
Positive Control
HL-60 or THP-1 cells. Lymph node and tonsil.
Immunogen
Stimulated human leukocytes
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).
Key References
  • Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1546-1547, 1578-1579.
  • C.L. Holness, et al, (1995) J Biol Chem 270: 877-884.
  • Y. van Kooyk, et al, (1996) J Exp Med 183: 1247-1252.
  • Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 403-409.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3385
SwissProt
P32942
Chromosome Location
19p13.2
Mol. Weight of Antigen
110-160kDa
Alternate Names
Intercellular adhesion molecule 3, CDw50, ICAM-R, ICAMR; Intercellular adhesion molecule 3 (ICAM3)

Summary

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

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