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A20 / TNFAIP3 (Marker of Tamoxifen Resistance) Antibody [TNFAIP3/2813]

Additional information

Catalog Number: 7128-MSM3
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7128-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7128-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7128-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
A20 / TNFAIP3 (Marker of Tamoxifen Resistance) Antibody

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).

A20 / TNFAIP3 (Marker of Tamoxifen Resistance) Antibody - Image 2

Formalin-fixed, paraffin-embedded human breast carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).

A20 / TNFAIP3 Antibody in SDS-PAGE

A20 / TNFAIP3 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified A20 / TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
TNFAIP3/2813
Isotype
IgG2c
Light Chain
Kappa
Cellular Localization
Cytoplasm, Lysosome, Nucleus
Gene Name
TNFAIP3
Positive Control
Hodgkin s Lymphoma or hepatocellular carcinoma (HCC).
Immunogen
Recombinant fragment (around aa 241-356) of human TNFAIP3 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of pro-inflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Key References
  • Schmitz R, et al. TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma. J Exp Med. 2009 May 11; 206(5):981-9.
  • J A Vendrell,et al. A20/TNFAIP3, a new estrogen-regulated gene that confers tamoxifen resistance in breast cancer cells. Oncogene,26:4656-4667; 2007.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7128
SwissProt
P21580
Chromosome Location
6q23
Mol. Weight of Antigen
~90kDa
Alternate Names
Tumor necrosis factor alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, A20; Putative DNA-binding protein A20; TNF alpha-induced protein 3 (TNFAIP3); Tumor Necrosis Factor alpha-Induced Protein 3(TNFAIP3); Zinc finger protein A20

Summary

A20 (also known as TNFAIP3) is a zinc finger protein and inhibits NF-kappa B activation as well as TNF-mediated apoptosis. The protein has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. A20 is as a tumor suppressor and has been shown to protect MCF-7 breast carcinoma cells from TNF-induced apoptosis. HighA20expression levels were observed in more aggressive breast tumors (ER-negative, progesterone receptor-negative and high histological grade). These findings strongly suggest that A20 is a key protein involved in tamoxifen resistance.

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