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Recombinant Endoglin / CD105 (Angiogenesis Marker) Antibody [ENG/13728R]

In Stock
Catalog Number Formulation Size Price
2022-RBM25-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2022-RBM25-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2022-RBM25-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD105 Antibody in Western Blot (WB)

CD105 Antibody in Western Blot (WB)

Western blot analysis of human kidney tissue lysate using CD105 Recombinant Rabbit Monoclonal Antibody (ENG/13728R).

CD105 Antibody in Western Blot (WB)

CD105 Antibody in Western Blot (WB)

Western blot analysis of kidney tissue lysates of different species using CD105 Recombinant Rabbit Monoclonal Antibody (ENG/13728R).

CD105 / Endoglin Antibody in SDS-PAGE

CD105 / Endoglin Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD105 Recombinant Rabbit Monoclonal Antibody (ENG/13728R) of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
ENG
Positive Control
Kidney.
Immunogen
Recombinant fragment (around aa345-365) of the human CD105 protein (exact sequence is proprietary)
Alternate Names
Endoglin, END|HHT1|ORW1

Database Links

Entrez Gene ID
2022
SwissProt
P17813

Additional Information

Clone
ENG/13728R
Chromosome Location
9q34.11
Mol. Weight of Antigen
70.58kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All ENG Antibodies

Functions

  • Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:21737454, PubMed:22347366, PubMed:23300529, PubMed:8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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