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Recombinant MCAM (Melanoma Cell Adhesion Molecule) / MUC18 / CD146 Antibody [MCAM/7513R]

In Stock
Catalog Number Formulation Size Price
4162-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4162-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4162-RBM13-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD146 / MUC18 / MCAM Antibody in Western Blot (WB)

CD146 / MUC18 / MCAM Antibody in Western Blot (WB)

Western blot analysis of SK-MEL-2 and SK-MEL-28 cell lysates using CD146 / MUC18 / MCAM Recombinant Rabbit Monoclonal Antibody (MCAM/7513R).

CD146 / MUC18 / MCAM Antibody in Western Blot (WB)

CD146 / MUC18 / MCAM Antibody in Western Blot (WB)

Western blot analysis of A-375 cell lysate using CD146 / MUC18 / MCAM Recombinant Rabbit Monoclonal Antibody (MCAM/7513R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Membrane
Gene Name
MCAM
Positive Control
Human skin or melanoma tissue (IHC). SK-MEL-2, SK-MEL-28, A-375
Immunogen
Recombinant fragment (around aa628-640) of the human CD146 protein (exact sequence is proprietary)
Alternate Names
Cell surface glycoprotein MUC18, CD146|HEMCAM|METCAM|MUC18|MelCAM

Database Links

Entrez Gene ID
4162
SwissProt
P43121

Additional Information

Clone
MCAM/7513R
Chromosome Location
11q23.3
Mol. Weight of Antigen
71.61kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All MCAM Antibodies

Functions

  • Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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