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Recombinant Myelin Basic Protein Antibody [rMBP/9301]

Additional information

Catalog Number: 4155-MSM12
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4155-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4155-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4155-MSM12-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Myelin Basic Protein Antibody

Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP/9301). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Myelin Basic Protein Antibody - Image 2

SDS-PAGE AnalysisPurified MBP Recombinant Mouse Monoclonal Antibody (rMBP/9301).Confirmation of Purity and Integrity of Antibody.

MBP Antibody in Western Blot (WB)

MBP Antibody in Western Blot (WB)

Western Blot Analysis of human brain tissue lysate using Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP/9301).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rMBP/9301
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Myelin membrane, Nucleus
Gene Name
MBP
Positive Control
Human brain or astrocytoma tissue.
Immunogen
Recombinant fragment (around aa82-87) of the human Myelin Basic protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4155
SwissProt
P02686
Chromosome Location
18q23
Mol. Weight of Antigen
33.12kDa
Alternate Names
Myelin basic protein, -

Summary

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. rMBP/9301 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. rMBP/9301 is used in a two-site ELISA with clone MBP/4273. Both are used interchangeably as capture and detection antibody.

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