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SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody [SDHB/3745]

Additional information

Catalog Number: 6390-MSM5
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6390-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6390-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6390-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody

Formalin-fixed, paraffin-embedded human liver stained with SDHB Mouse Monoclonal Antibody (SDHB/3745). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified SDHB Mouse Monoclonal Antibody (SDHB/3745). Confirmation of Purity and Integrity of Antibody.

  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Clone
SDHB/3745
Isotype
IgG2
Light Chain
Kappa
Cellular Localization
Cytoplasm. Mitochondria.
Gene Name
SDHB
Positive Control
Jurkat or HepG2 cells. Human kidney or liver.
Immunogen
Recombinant human SDHB fragment around aa 165-273 (Exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Key References
  • Allibhai, Z., et al. 2004. Malignant pheochromocytoma associated with germline mutation of the SDHB gene. J. Urol. 172: 1409-1410.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6390
SwissProt
P21912
Chromosome Location
1p36.13
Mol. Weight of Antigen
32kDa
Alternate Names
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Iron-sulfur subunit of complex II, CWS2; DHSB; Iron-sulfur subunit of complex II; PGL4; SDH2; SDHB; SDHIP; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein

Summary

Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. �Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. �Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST. �

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