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CD3e (T-Cell Marker) Antibody [OKT3]

Additional information

Catalog Number: 916-MSM4
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
916-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
916-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
916-MSM4-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD3e (T-Cell Marker) Antibody

SDS-PAGE Analysis of Purified CD3e Mouse Monoclonal Antibody (OKT3). Confirmation of Purity and Integrity of Antibody.

CD3e (T-Cell Marker) Antibody - Image 2

Flow cytometry of lymphocyte gated PBMCs stained with CD3 mouse monoclonal antibody (OKT3) (red) or isotype control (gray) followed by goat anti-mouse CF640R (red)

  • Flow
  • Functional Studies
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Clone
OKT3
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CD3E
Positive Control
Jurkat cells or PBMCs. Tonsil and lymph node
Immunogen
Human PBMC s
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Key References
  • Haga-Friedman A, et al. Jun 2012. J. Immunol. 188:5538-5546. (in vitro activation)
  • Bagnara D, et al. 2011. Blood. 117: 5463-5472. (in vivo depletion)
  • Roura-Mir C, et al. 2005. J. Immunol. 174:3773-3780 (IHC Acetone fixed frozen sections)
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
916
SwissProt
P07766
Chromosome Location
11q23.3
Mol. Weight of Antigen
23kDa
Alternate Names
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3 epsilon, CD3 TCR complex, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

Summary

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.

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