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Thyroglobulin (Thyroidal Cell Marker) Antibody [2H11 + 6E1]

Additional information

Catalog Number: 7038-MSM3
Reactivity:

, ,

Gene:

Host:

In Stock

Trial Size Available

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Best Seller
Catalog Number Formulation Size Price
7038-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7038-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7038-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Thyroglobulin (Thyroidal Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).

  • Flow
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunohistochemistry
Species Reactivity
Human, Mouse, Rat
Clone
2H11 + 6E1
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Secreted
Gene Name
TG
Positive Control
Thyroid.
Immunogen
Human thyroid follicular cells
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (PubMed:32025030, PubMed:17532758). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen (PubMed:32025030). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (PubMed:32025030). One dimer produces 7 thyroid hormone molecules (PubMed:32025030).
Key References
  • Judkins AR et al. Hum Pathol. 1999;30(11):1373-6
  • Bellet, D, et al. J Clin Endocrin Metab 1983;56:530-533
  • Ossendorp FA, et. al. Journal of Immunological Methods, 1989, 120(2):191-200.
  • Heffess CS et al. Cancer. 2002;95(9):1869-78
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7038
SwissProt
P01266
Chromosome Location
8q24.22
Mol. Weight of Antigen
660kDa (Dimeric Form)
Alternate Names
Thyroglobulin, AITD3; hTG; TDH3; Tg; Tgn

Summary

Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

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