MED22 (Transcription Factor) Antibody [PCRP-MED22-1E4]

These antibodies are validated on HuProtâ„¢ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 6837-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
6837-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6837-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6837-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • ELISA
  • Flow
  • IF
  • WB

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Host
Mouse
Species Reactivity
Clone
PCRP-MED22-1E4
Isotype
IgG2c
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa or U87 cells.
Immunogen
Recombinant full-length human MED22 protein
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
SwissProt
Chromosome Location
9q34.2
Mol. Weight of Antigen
22kDa
Alternate Names
Mediator of RNA polymerase II transcription subunit 22, Mediator complex subunit 22, Surfeit locus protein 5, Med24; SURF5; mediator complex subunit 22; Mediator of RNA polymerase II transcription subunit 22

Summary

Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.

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