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CD45RO (T-Cell Marker) Antibody [UCHL-1]

Additional information

Catalog Number: 5788-MSM1
Reactivity:

, ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5788-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5788-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5788-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD45RO (T-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1).

CD45RO (T-Cell Marker) Antibody - Image 2

Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).

CD45RO (T-Cell Marker) Antibody - Image 3

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Monoclonal Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

CD45RO (T-Cell Marker) Antibody - Image 4

SDS-PAGE Analysis of Purified CD45RO MAb (UCHL-1). Confirmation of Integrity and Purity of Antibody

CD45RO (T-Cell Marker) Antibody - Image 5

Immunofluorescence Analysis of human tonsil FFPE section stained with anti-CD45RO (UCHL-1) and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.

PTPRC Antibody in SDS-PAGE

PTPRC Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Receptor-type tyrosine-protein phosphatase C Mouse Monoclonal Antibody (UCHL-1). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Chimpanzee, Common Marmoset, Human
Clone
UCHL-1
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell membrane, Membrane raft
Gene Name
PTPRC
Positive Control
CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
  • (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Key References
  • Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138.3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5788
SwissProt
P08575
Chromosome Location
1q31.3
Mol. Weight of Antigen
180-185kDa
Alternate Names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Summary

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

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