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Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 Mouse Monoclonal Antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using UBR2 Mouse Monoclonal Antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
SDS-PAGE Analysis UBR2 Mouse Monoclonal Antibody (PCRP-UBR2-1D12). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human thyroid stained with UBR2 Mouse Monoclonal Antibody (PCRP-UBR2-1D12) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Analysis of Protein Array containing more than 19,000 full-length human proteins using UBR2-Monospecific Mouse Monoclonal Antibody (PCRP-UBR2-1D12). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28.
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