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Collagen VII Antibody [LH7.2]

Additional information

Catalog Number: 1294-MSM1
Reactivity:

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Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1294-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1294-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1294-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Collagen VII Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).

Collagen VII Antibody - Image 2

Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).

Collagen VII Antibody - Image 3

SDS-PAGE Analysis of Purified Collagen VII Mouse Monoclonal Antibody (LH7.2). Confirmation of Purity and Integrity of Antibody.

Collagen VII Antibody - Image 4

Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VII using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is RedDot.

Collagen VII Antibody - Image 5

Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

  • ELISA
  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Goat, Guinea Pig, Horse, Human, Sheep
Clone
LH7.2
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
COL7A1
Positive Control
cells. Human lymphocytes. Human lymph node or tonsil., HeLa
Immunogen
Recombinant full-length human Collagen VII protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Key References
  • Ponsioen TL et al. Mol Vis 14:652-60 (2008).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1294
SwissProt
Q02388
Chromosome Location
3p21.31
Mol. Weight of Antigen
290kDa
Alternate Names
Collagen alpha-1(VII) chain, Long-chain collagen, Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1(VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen

Summary

Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

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