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Recombinant CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) Antibody [CDX2/2951R]

Additional information

Catalog Number: 1045-RBM5
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1045-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1045-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1045-RBM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CDX2 Rabbit Recombinant Monoclonal Antibody (CDX2/2951R).

Recombinant CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CDX2 Rabbit Recombinant Monoclonal Antibody (CDX2/2951R).

CDX2 Antibody in Western Blot (WB)

CDX2 Antibody in Western Blot (WB)

Western blot analysis of HEK293T, human colon and human small intestine lysates using CDX2 Recombinant Rabbit Monoclonal Antibody (CDX2/2951R).

CDX2 Antibody in SDS-PAGE

CDX2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Homeobox protein CDX-2 Rabbit Monoclonal Antibody (CDX2/2951R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CDX2/2951R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
CDX2
Positive Control
HT29 cells. Colon Carcinoma. small intestine, HEK293T
Immunogen
Recombinant fragment (around aa150-249) of the human CDX2 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1045
SwissProt
Q99626
Chromosome Location
13q12.2
Mol. Weight of Antigen
33.52kDa
Alternate Names
Homeobox protein CDX-2, CDX-3|CDX2/AS|CDX3

Summary

The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoidsand is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma.However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

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