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TGF-alpha (Transforming Growth Factor alpha) Antibody [P/T1]

In Stock
Catalog Number Formulation Size Price
7039-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7039-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7039-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Monoclonal Antibody (P/T1)

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Monoclonal Antibody (P/T1)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Rabbit, Zebrafish
Research Areas
Cardiovascular, AKT Signaling, Angiogenesis, Infectious Disease, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Extracellular space, Secreted
Gene Name
TGFA
Positive Control
Jurkat or Ramos cells. Testicular Carcinoma.
Immunogen
A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.
Alternate Names
Protransforming growth factor alpha, EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1

Database Links

Entrez Gene ID
7039
SwissProt
P01135

Additional Information

Clone
P/T1
Chromosome Location
2p13.3
Mol. Weight of Antigen
6kDa

Related Antibodies

View All TGFA Antibodies

Functions

  • TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Key References

  • Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35.
  • Nasim, M. M., Thomas, D. M., Alison, M. R., and Fil]ipe, M. I. Transforming growth factor a expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma - an immunohistochemical study, Histopathology. 20: 339-343, 1992.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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