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SATB1 (Marker of Lymph Node Metastasis of CRC) Antibody [PCRP-SATB1-2C3]

In Stock
Catalog Number Formulation Size Price
6304-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6304-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6304-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow cytometric analysis of PFA-fixed HeLa cells. SATB1 Mouse Monoclonal Antibody (PCRP-SATB1-2C3) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Flow cytometric analysis of PFA-fixed HeLa cells. SATB1 Mouse Monoclonal Antibody (PCRP-SATB1-2C3) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

SDS-PAGE Analysis of Purified SATB1 Mouse Monoclonal Antibody (PCRP-SATB1-2C3). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified SATB1 Mouse Monoclonal Antibody (PCRP-SATB1-2C3). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SATB1 Mouse Monoclonal Antibody (PCRP-SATB1-2C3) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SATB1 Mouse Monoclonal Antibody (PCRP-SATB1-2C3) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Isotype / Light Chain
IgG /
Cellular Localization
Nucleus.
Gene Name
SATB1
Positive Control
HeLa cells.
Immunogen
Recombinant full-length human SATB1 protein
Alternate Names
DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1, DNA-binding protein SATB1; SATB family member 1; SATB homeobox 1; SATB1; Special AT-rich sequence-binding protein 1

Database Links

Entrez Gene ID
6304
SwissProt
Q01826

Additional Information

Clone
PCRP-SATB1-2C3
Chromosome Location
3p24.3
Mol. Weight of Antigen
115kDa

Related Antibodies

View All SATB1 Antibodies

Functions

  • Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis. Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone, possibly by positively regulating the expression of NEUROD1 (By similarity).

Key References

  • Baba, H., et al. Anticancer Research 2016, 36(8):4069-76.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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