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  • HuProt™ Validated, PCRP

SAMD4B (Transcription Factor) Antibody [PCRP-SAMD4B-1H3]

These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 55095-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
55095-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
55095-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
55095-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
How do I understand the HuProtTM Validation Results? Learn More
SAMD4B (Transcription Factor) Antibody

Flow cytometric analysis of PFA-fixed HeLa cells. SAMD4B Mouse Monoclonal Antibody (PCRP-SAMD4B-1H3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SAMD4B (Transcription Factor) Antibody - Image 2

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SAMD4B Mouse Monoclonal Antibody (PCRP-SAMD4B-1H3)

SAMD4B (Transcription Factor) Antibody - Image 3

followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

SAMD4B (Transcription Factor) Antibody - Image 4

SDS-PAGE Analysis SAMD4B Mouse Monoclonal Antibody (PCRP-SAMD4B-1H3). Confirmation of Purity and Integrity of Antibody.

  • ELISA
  • Flow
  • IF
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Clone
PCRP-SAMD4B-1H3
Isotype
IgG2c
Cellular Localization
Cytoplasm, Nucleus
Gene Name
SAMD4B
Positive Control
HeLa cells.
Immunogen
Recombinant full-length humanSAMD4Bprotein
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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MICA Antibody

Additional information

Catalog Number: 100507436-MSM3
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
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MICA Antibody

Additional information

Catalog Number: 100507436-MSM2
Reactivity:

, , , ,

Gene:

Host:

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Ksp-Cadherin / CDH16 (Renal Cell Marker) Antibody

Additional information

Catalog Number: 1014-MSM6
Reactivity:

Gene:

Host:

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Additional information

Catalog Number: 1014-MSM5
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).
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Additional information

Catalog Number: 1014-MSM1
Reactivity:

, , , ,

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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HDAC6 Antibody

Additional information

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Reactivity:

Gene:

Host:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Has transcriptional repressor activity. Overexpression inhibits the transcriptional activities of AP-1, p53/TP53 and CDKN1A.
Key References
  • Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.
  • Luo N, et al.SAMD4B, a novel SAM-containing protein, inhibits AP-1-, p53- and p21-mediated transcriptional activity. BMB Rep, 2010 May. PMID 20510020
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
55095
SwissProt
Q5PRF9
Chromosome Location
19q13.2
Mol. Weight of Antigen
75.48kDa
Alternate Names
Protein Smaug homolog 2, Sterile alpha motif domain-containing protein 4B, Sterile alpha motif domain containing 4B; hSmaug2; MGC99832; SMGB

Summary

Has transcriptional repressor activity. Overexpression inhibits the transcriptional activities of AP-1, p53/TP53 and CDKN1A.

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