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Recombinant TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Antibody [rTIMP2/2335]

In Stock
Catalog Number Formulation Size Price
7077-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7077-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7077-MSM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Antibody

Formalin-fixed, paraffin-embedded human Placenta stained with TIMP2 Mouse Recombinant Monoclonal Antibody (rTIMP2/2335).

Recombinant TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Antibody - Image 2

SDS-PAGE Analysis of Purified TIMP2 Mouse Recombinant Monoclonal Antibody (rTIMP2/2335). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
TIMP2
Positive Control
Colon, Endometrial, HeLa cells. Normal Placenta. Breast, Prostate or Ovarian Carcinoma.
Immunogen
Recombinant fragment corresponding to the n-terminal region of the human TIMP2 protein
Alternate Names
Metalloproteinase inhibitor 2, CSC-21K|DDC8

Database Links

Entrez Gene ID
7077
SwissProt
P16035

Additional Information

Clone
rTIMP2/2335
Chromosome Location
17q25.3
Mol. Weight of Antigen
24.4kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All TIMP2 Antibodies

Functions

  • Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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