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Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody [rKIP1/1356]

Additional information

Catalog Number: 1027-MSM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1027-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1027-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1027-MSM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody

SDS-PAGE Analysis of Purified p27 Recombinant Mouse Monoclonal Antibody (rKIP1/1356). Confirmation of Purity and Integrity of Antibody.

Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody - Image 2

Formalin-fixed, paraffin-embedded human breast carcinoma stained with p27 Recombinant Mouse Monoclonal Antibody (rKIP1/1356). Inset: PBS instead of primary antibody. Secondary negative control.

p27 Antibody in Western Blot (WB)

p27 Antibody in Western Blot (WB)

Western blot analysis of Human skeletal muscle, Human spleen, A431 and HeLa lysates using p27 Recombinant Mouse Monoclonal Antibody (rKIP1/1356).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rKIP1/1356
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm, Endosome, Nucleus
Gene Name
CDKN1B
Positive Control
Breast, Cervical or Colon Carcinoma., HeLa or MCF7, A431, 3T3 cells. Skeletal muscle, tonsil
Immunogen
Recombinant full-length human CDKN1B protein
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1027
SwissProt
P46527
Chromosome Location
12p13.1
Mol. Weight of Antigen
22.07kDa
Alternate Names
Cyclin-dependent kinase inhibitor 1B, CDKN4|KIP1|MEN1B|MEN4|P27KIP1

Summary

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- � � induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

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