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Recombinant Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Antibody [AAT/3167R]

In Stock
Catalog Number Formulation Size Price
5265-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5265-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5265-RBM4-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Antibody

SDS-PAGE Analysis of Purified Alpha-1-Antitrypsin Recombinant Rabbit Monoclonal (AAT/3167R). Confirmation of Purity and Integrity of Antibody.

Recombinant Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Breast stained with Alpha-1-Antitrypsin Recombinant Rabbit Monoclonal Antibody (AAT/3167R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Antibody - Image 3

Western Blot Analysis of Jurkat and A549 cell lysates using Alpha-1-Antitrypsin Recombinant Rabbit Monoclonal (AAT/3167R).

SERPINA1 Antibody in Western Blot (WB)

SERPINA1 Antibody in Western Blot (WB)

Western blot analysis of human Lung tissue lysate using SERPINA1 Recombinant Rabbit Monoclonal Antibody (AAT/3167R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Endoplasmic reticulum, Extracellular matrix, Extracellular space, Secreted
Gene Name
SERPINA1
Positive Control
A549 or HepG2 cells. Tonsil, hepatocellular carcinoma or histiocytoma (IHC)., Jurkat, Lung
Immunogen
Recombinant fragment (around aa49-187) of the human Alpha-1-Antitrypsin protein (exact sequence is proprietary)
Alternate Names
Alpha-1-antitrypsin, A1A|A1AT|AAT|PI|PI1|PRO2275|alpha1AT|nNIF

Database Links

Entrez Gene ID
5265
SwissProt
P01009

Additional Information

Clone
AAT/3167R
Chromosome Location
14q32.13
Mol. Weight of Antigen
46.74kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All SERPINA1 Antibodies

Functions

  • Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin., Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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