Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

NR3C2 Antibody [PCRP-NR3C2-1E3]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
4306-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4306-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4306-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells

Summary

Mineralocorticoid hormones are primarily found in epithelial tissues where they function as regulators of Na+, K+ and H+ ion transport. Aldosterone is a mineralocorticoid that has been shown to regulate electrolyte excretion and intravascular volume and is therefore involved in blood pressure regulation. Mineralocorticoid receptor (MCR or MR) is a member of the steroid/thyroid/ retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor downregulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Nucleus.
Gene Name
Positive Control
HeLa cells.
Immunogen
Recombinant fragment (around aa601-673) of human NR3C2 protein
Alternate Names
Mineralocorticoid receptor, Nuclear receptor subfamily 3 group C member 2, MLR; MR

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-NR3C2-1E3
Chromosome Location
4q31.23
Mol. Weight of Antigen
107kDa

Functions

  • Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

Key References

  • Blackshaw S, et al. Nature methods15.5 (2018 May): 330-338.
  • Statement for PublicationAll investigators should include an acknowledgment in the publications the benefit from the use of this product. Following statement is suggested: PCRP-NR3C2-1E3 was developed by JHU/CDI under Protein Capture Reagents Program of NICHD of the NIH.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “NR3C2 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK