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MYH7 Antibody [MYH7/9183]

These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.

The MAbMan recommends these antibodies for repeatable and reliable results.

Additional information

Catalog Number: 4625-MSM2
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4625-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4625-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4625-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MYH7 Antibody

Formalin-fixed, paraffin-embedded human skeletal muscle stained with MYH7 Mouse Monoclonal Antibody (MYH7/9183). Inset: PBS instead of primary antibody; secondary only negative control.

MYH7 Antibody - Image 2

SDS-PAGE Analysis of Purified MYH7 Mouse Monoclonal Antibody (MYH7/9183). Confirmation of Purity and Integrity of Antibody.

Myosin Heavy Chain 7 Antibody in Western Blot (WB)

Myosin Heavy Chain 7 Antibody in Western Blot (WB)

Western Blot Analysis of human heart tissue lysate using Myosin Heavy Chain 7 Mouse Monoclonal Antibody (MYH7/9183).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
MYH7/9183
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cytoplasm > myofibril. Thick filaments of the myofibrils.
Gene Name
MYH7
Positive Control
Skeletal muscle. Heart.
Immunogen
Recombinant fragment (around aa1150-1350) of human MYH7 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Host:

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Additional information

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Host:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
Key References
  • Bian, Z.Y., et al. 2012. Disruption of mindin exacerbates cardiac hypertrophy and fibrosis. J. Mol. Med. 90: 895-910.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4625
SwissProt
P12883
Chromosome Location
14q11.2
Mol. Weight of Antigen
223kDa
Alternate Names
Myosin-7, Myosin heavy chain 7, Myosin heavy chain slow isoform, Myosin heavy chain, cardiac muscle beta isoform, Beta myosin heavy chain; cardiac muscle beta isoform; CMD1S; CMH1; MPD1; MyHC-beta; MyHC-slow; MYHCB; Myopathy, distal 1; Myosin heavy chain (AA 1-96); Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle beta isoform; Myosin, heavy chain 7, cardiac muscle, beta; Myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; Rhabdomyosarcoma antigen MU RMS 40.7A; SPMD; SPMM

Summary

Myosin heavy chains are ubiquitous Actin-based motor proteins that convert the chemical energy derived from ATP hydrolysis into the mechanical energy that drives diverse motile processes in eukaryotic cells, including cytokinesis, vesicular transport and cellular locomotion. Muscle myosin is a heterohexamer consisting of two myosin heavy chains and two associated nonidentical pairs of myosin light chains. The seven myosin heavy chain isoforms that predominate in mammalian skeletal muscles include two developmental isoforms, MHC-embryonic (MYH3) and MHC-perinatal (MYH8); three adult skeletal muscle isoforms, MHC IIa (MYH2), MHC IIb (MYH4) and MHC IIx/d (MYH1); and MHC-β/slow (MYH7 or MHC-β), which is also expressed in cardiac muscle. Research indicates that mutations of the MYH7 gene causes hypertrophic cardiomyopathy.

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