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MED21 / SRB7 Antibody [PCRP-MED21-4B5]

In Stock
Catalog Number Formulation Size Price
9412-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
9412-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
9412-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml

Summary

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa or K562 cells.
Immunogen
Recombinant full-length human MED21 protein
Alternate Names
Mediator of RNA polymerase II transcription subunit 21, Mediator complex subunit 21, RNA polymerase II holoenzyme component SRB7, Forkhead box protein Q1; MED21; Hepatocyte nuclear factor 3 forkhead homolog 1; HFH1; HNF3/forkhead like protein 1; MGC77469; Q1; Winged helix / forkhead transcription factor; zgc:77469

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-MED21-4B5
Chromosome Location
12p11.23
Mol. Weight of Antigen
15.5kDa

Functions

  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Key References

  • Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.
  • Nucleic Acids Res. 38:D142-D148 (2010).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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