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SDS-PAGE Analysis Purified HK1 Mouse Monoclonal Antibody (HK1/9509). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human brain stained with Hexokinase 1 Mouse Monoclonal Antibody (HK1/9509). Inset: PBS instead of primary antibody; secondary only negative control.
Western blot analysis of human brain tissue lysate using HK1 Mouse Monoclonal Antibody (HK1/9509).
Western blot analysis of human kidney, human skeletal muscle, human skin and U87 lysates using HK1 Mouse Monoclonal Antibody (HK1/9509).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
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