SDS-PAGE Analysis Purified Follistatin Mouse Monoclonal Antibody (FST/4281). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified Follistatin Mouse Monoclonal Antibody (FST/4281). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human testis stained with Follistatin Mouse Monoclonal Antibody (FST/4281).

Formalin-fixed, paraffin-embedded human testis stained with Follistatin Mouse Monoclonal Antibody (FST/4281).

Printable Datasheet

Follistatin / Activin Binding Protein [FST/4281]

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Summary

Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4281 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody FST/4282 in a two site ELISA for the detection of Follistatin.

Product Properties & Targets

Host
Mouse
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Isotype
IgG1
Cellular Localization
Secreted
Gene Name
FST
Positive Control
Human testis or ovary.
Immunogen
Raised to human Follistatin (Fst) 288 and recognizes both human Fst 288 and 315
Alternate Names
Follistatin, Activin-binding protein, Follistatin, FS, FST, Active-binding protein

Database Links

Entrez Gene ID
10468
Unigene
9914
SwissProt
P19883

Additional Information

Clone
FST/4281
Human Gene Symbol
FST
Chromosome Location
5q11.2
Mol. Weight of Antigen
37kDa

Functions

  • Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).

Known Applications & Suggested Dilutions

  • ELISA (Use in combination with clone FST/4282 for two-site ELISA)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Evans, L. (1998). Journal of Endocrinology 156.2; 275-82.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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