SDS-PAGE Analysis Purified Follistatin Mouse Monoclonal Antibody (FST/4282). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified Follistatin Mouse Monoclonal Antibody (FST/4282). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

Follistatin / Activin Binding Protein [FST/4282]

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Summary

Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4282 recognizes recombinant human Follistatin 288, allowing for detection of FSH levels using various analysis methods. This antibody also works in a two site ELISA with FST/4281.

Product Properties & Targets

Host
Mouse
Applications
ELISA, Western Blot
Species Reactivity
Human
Isotype
IgG1
Cellular Localization
Secreted
Gene Name
FST
Positive Control
Human testis or ovary.
Immunogen
Recognizes human Follistatin isoform FS288, raised against recombinant human FS288.
Alternate Names
Follistatin, Activin-binding protein, Follistatin, FS, FST, Active-binding protein

Database Links

Entrez Gene ID
10468
Unigene
9914
SwissProt
P19883

Additional Information

Clone
FST/4282
Human Gene Symbol
FST
Chromosome Location
5q11.2
Mol. Weight of Antigen
37kDa

Functions

  • Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).

Known Applications & Suggested Dilutions

  • ELISA (1ug/ml)
  • Immunocytochemistry (70ug/ml)
  • Western Blot (1-2ug/ml)
  • ,Optimal dilution for a specific application should be determined.

Key References

  • Evans, L. (1998). Journal of Endocrinology 156.2; 275-82.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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