SDS-PAGE Analysis Purified Clathrin, HC Monoclonal Antibody (CHC/1432). Confirmation of Purity and Integrity of Antibody.

Confocal Immunofluorescence image of HeLa cells stained with Clathrin, HC Monoclonal Antibody (CHC/1432) followed by Goat anti-Mouse CF488 (green). Reddot is used to label the nuceli red.

Printable Datasheet

Clathrin, Heavy Chain [CHC/1432]

Name: Clathrin, Heavy Chain
SKU: 1213-MSM2
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.

Product Properties & Targets

Host

Mouse

Species Reactivity

African Green Monkey, Bird, Cow, Dog, Human, Mouse, Pig, Rat, Xenopus Laevis

Isotype

IgG1

Cellular Localization

Coated pit, Cytoplasmic vesicle membrane, Membrane

Light Chain

kappa

Gene Name

CLTCL1

Positive Control

HeLa cells. Placenta or Prostate Carcinoma.

Immunogen

Recombinant full-length human Clathrin Heavy Chain protein

Alternate Names

Clathrin heavy chain 2, Clathrin heavy chain on chromosome 22, Clathrin heavy chain; Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17 (CHC17 or CHC-17); CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD

Database Links

Entrez Gene ID

1213

Unigene

491351

SwissProt

P53675; Q00610

Additional Information

Clone

CHC/1432

Human Gene Symbol

CLTC

Chromosome Location

17q23.1

Mol. Weight of Antigen

192kDa

Functions

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).

Known Applications & Suggested Dilutions

Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Key References

Pearse, B.M. 1987. Clathrin and coated vesicles. EMBO J. 6: 2507-2512.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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