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Cdk2 / p34cdc2 Serine-Threonine Kinase Antibody [AN4.3]

Additional information

Catalog Number: 1017-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1017-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
1017-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1017-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CDK2 Antibody in Western Blot (WB)

CDK2 Antibody in Western Blot (WB)

Western blot analysis of Jurkat and HEK293T cell lysates using CDK2 Mouse Monoclonal Antibody (AN4.3).

CDK2 Antibody in Western Blot (WB)

CDK2 Antibody in Western Blot (WB)

Western blot analysis of 293T and K-562 cell lysates using CDK2 Mouse Monoclonal Antibody (AN4.3).

CDK2 Antibody in SDS-PAGE

CDK2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CDK2 Mouse Monoclonal Antibody (AN4.3). Confirmation of Purity and Integrity of Antibody.

  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Western Blot
Species Reactivity
Human
Clone
AN4.3
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cajal body, Centrosome, Cytoplasm, Cytoskeleton, Endosome, Microtubule organizing center, Nucleus
Gene Name
CDK2
Positive Control
Jurkat, HEK293T, K-562 or HeLa cells.
Immunogen
Recombinant full-length human Cdk2 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:12944431). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878).
Key References
  • Hafeez, B.B., et al. 2008. A dietary anthocyanidin delphinidin induces apoptosis of human prostate cancer PC3 cells in vitro and in vivo: involvement of nuclear factor kB signaling. Cancer Res. 68: 8564-8572.
  • Xu, Z.W., et al. 2010. Targeting the Na+/K+-ATPase a1 subunit of hepatoma Hep G2 cell line to induce apoptosis and cell cycle arresting. Biol. Pharm. Bull. 33: 743-751.
  • Napione, L., et al. 2012. IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21Cip1/Waf1-mediated mechanism. Angiogenesis 15: 713-725.
  • Liu, Z., et al. 2013. CASZ1 inhibits cell cycle progression in neuroblastoma by restoring pRb activity. Cell Cycle 12: 2210-2218.
  • Seoane, M., et al. 2019. Lineage-specific control of TFIIH by MITF determines transcriptional homeostasis and DNA repair. Oncogene 38: 3616-3635.
  • Zhou, Y., et al. 2020. The requirement for cyclin E in c-Myc overexpressing breast cancers. Cell Cycle 19: 2589-2599.
  • Yeung, T.K., et al. 2021. One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines. Mol. Biol. Cell 32: 1320-1330.
  • Lau, H.W., et al. 2021. Quantitative differences between cyclin-dependent kinases underlie the unique functions of CDK1 in human cells. Cell Rep. 37: 109808.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1017
SwissProt
P24941
Chromosome Location
12q13.2
Mol. Weight of Antigen
34kDa
Alternate Names
Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, Cdc2 related protein kinase; CDC28; CDC2A; CDK1; CDK2; CDKN2; Cell devision kinase 2; Cell division protein kinase 2; cyclin dependent kinase 2 alpha; kinase Cdc2; MPF; p33 protein kinase

Summary

In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin dependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE.

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