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CD70 / TNFSF7 (Activated T- & B-Lymphocyte Marker) Antibody [BU69]

In Stock
Catalog Number Formulation Size Price
970-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
970-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
970-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
Positive Control
Raji cells. Lymph node or tonsil.
Immunogen
Human WM-1 (Waldenstrom s macroglobulinemia) cell line
Alternate Names
CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27 ligand (CD27L); CD27LG; Ki24; Surface antigen CD70; Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

Database Links

Entrez Gene ID
970
SwissProt

Additional Information

Clone
BU69
Chromosome Location
19p13
Mol. Weight of Antigen
30kDa

Functions

  • Cytokine which is the ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Key References

  • Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138.
  • R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773.
  • M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761.
  • K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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