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CD284 (Toll-Like Receptor 4) Antibody [TLR4/230]

In Stock
Catalog Number Formulation Size Price
7099-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7099-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7099-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified CD284 Mouse Monoclonal Antibody (TLR4/230). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD284 Mouse Monoclonal Antibody (TLR4/230). Confirmation of Integrity and Purity of Antibody.

Summary

This MAb reacts with human Toll-like receptor 2 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Functional Studies
Species Reactivity
Dog, Guinea Pig, Human, Monkey, Pig, Rat
Research Areas
Apoptosis, Cardiovascular, Immuno Oncology, Immunology, Colon Cancer, Infectious Disease
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane, Cell projection, Early endosome, Ruffle
Gene Name
TLR4
Positive Control
HeLa, human peripheral blood cells. Dendritic cells, macrophage and monocytes in tonsil; placenta and adrenal., PC12
Immunogen
Recombinant full-length human TLR4 protein
Alternate Names
Toll-like receptor 4, hToll, ARMD10; CD284; hToll; TLR4; TOLL; Toll Endotoxin Hyporesponsiveness; Toll-like receptor 4

Database Links

Entrez Gene ID
7099
SwissProt
O00206

Additional Information

Clone
TLR4/230
Chromosome Location
9q33.1
Mol. Weight of Antigen
95-120kDa

Related Antibodies

View All TLR4 Antibodies

Functions

  • Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:27022195). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9237759, PubMed:10835634, PubMed:27022195,PubMed:21393102). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific (PubMed:20711192). Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed:15809303). In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor (PubMed:16622205, PubMed:10835634, PubMed:15809303, PubMed:17478729, PubMed:20037584, PubMed:20711192, PubMed:23880187, PubMed:27022195, PubMed:9237759). Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (PubMed:29038465).

Key References

  • Wei, X.Q., et al. 2008. Clin. Invest. Med. 31: E123-E130.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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