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Recombinant Arginase1 (Hepatocellular Carcinoma Marker) Antibody [ARG1/6790R]

Additional information

Catalog Number: 383-RBM13
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
383-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
383-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
383-RBM13-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Arginase-1 Antibody in Immunohistochemistry (IHC (P))

Arginase-1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase-1 Recombinant Rabbit Monoclonal Antibody (ARG1/6790R). Inset: PBS instead of primary antibody; secondary only negative control.

Arginase 1 Antibody in Western Blot (WB)

Arginase 1 Antibody in Western Blot (WB)

Western blot analysis of Human Liver, Mouse Liver, Rat Liver, Hamster Liver and Guinea pig Liver tissue lysates using Arginase-1 Recombinant Rabbit Monoclonal Antibody (ARG1/6790R).

Arginase-1 / ARG1 Antibody in SDS-PAGE

Arginase-1 / ARG1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Arginase-1 Recombinant Rabbit Monoclonal Antibody (ARG1/6790R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
ARG1/6790R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Cytoplasmic granule
Gene Name
ARG1
Positive Control
293T cells. Human liver or hepatocellular carcinoma (HCC).
Immunogen
Recombinant fragment (around aa222-322) of the human Arginase-1 protein (epitope: 309-322)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Host:

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Other Information

Functions
  • Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys., Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
383
SwissProt
P05089
Chromosome Location
6q23.2
Mol. Weight of Antigen
34.73kDa
Alternate Names
Arginase-1, -

Summary

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

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