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Catenin, gamma (Cardiomyocyte Marker) Antibody [[11E4]]

Additional information

Catalog Number: 3728-MSM1
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3728-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3728-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3728-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Catenin, gamma (Cardiomyocyte Marker) Antibody

Western Blot of HT20, A549, 293 and A431 cell lysates using C Catenin, gamma Mouse Monoclonal Antibody (11E4).

Catenin, gamma (Cardiomyocyte Marker) Antibody - Image 2

Confocal immunofluorescence image of HeLa cells using Catenin, gamma Mouse Monoclonal Antibody (11E4) Green (CF488) and Reddot is used to label the nuclei Red.

Catenin, gamma (Cardiomyocyte Marker) Antibody - Image 3

Western Blot Analysis of human HeLa and SKBR3 cell lysate using Catenin, gamma Mouse Monoclonal Antibody (11E4)

Catenin, gamma (Cardiomyocyte Marker) Antibody - Image 4

Flow Cytometric Analysis of PFA-fixed MCF cells. Catenin, gamma Mouse Monoclonal Antibody (11E4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Flow
  • IF
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human, Mouse
Clone
[11E4]
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Adherens junction, Cell junction, Cytoplasm, Cytoskeleton, Desmosome, Membrane
Gene Name
JUP
Positive Control
293 or A431 cells. Skin., A549, HeLa, HT20, SK-BR3
Immunogen
Recombinant full length human plakoglobin, fused to maltose binding protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
Key References
  • Sacco, PA; McGranahan, TM; Wheelock, MJ; Johnson, KR. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem 1995, 270:20201-6
  • Johnson, KR; Lewis, JE; Li, D; Wahl, J; Soler, AP; Knudsen, KA; Wheelock, MJ. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252-60
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3728
SwissProt
P14923
Chromosome Location
17q21
Mol. Weight of Antigen
80-87kDa
Alternate Names
Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3, ARVD12; Catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; CTNNG; Desmoplakin III; Desmoplakin-3; DP3; DPIII; Junction Plakoglobin; PDGB; PKGB

Summary

It recognizes a protein of 80-87kDa, identified as gamma-catenin. The catenins (, , and ) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. T-catenin binds to E-catenin as well as to -catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for -T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.

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