IL-4 (Interleukin-4), Mouse [11B11]

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Summary

IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4R, and dendritic cell IL-12.

Product Properties & Targets

Host
Rat
Species Reactivity
Isotype
Cellular Localization
Gene Name
IL4
Mol. Weight of Antigen
13-14kDa
Immunogen
Partially Purified native Mouse IL-4

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
11B11
Chromosome Location
11 B1.3; 11 31.97 cM (Mouse)

Known Applications & Suggested Dilutions

  • ELISA (For coating, order Ab without BSA & Azide)
  • Neutralization Studies (Order Ab without BSA & Azide)
  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Optimal dilution for a specific application should be determined.

Key References

  • Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  • Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA)
  • Hara M, et al. 2001. J. Immunol. 166:3789. (Neutralization)
  • Charles N, et al. 2010. Nat. Med. 16:701. (FC)
  • Wang W, et al. 2007. J. Immunol. 178:4885. (Neutralization)
  • Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
  • Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
  • Zavorotinskaya T, et al. 2003. Mol. Ther. 7:155. (IP)
  • Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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