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CD45RO (T-Cell Marker) Antibody [UCHL-1]

In Stock
Catalog Number Formulation Size Price
5788-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5788-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5788-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1).

Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).

Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Monoclonal Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Monoclonal Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

SDS-PAGE Analysis of Purified CD45RO MAb (UCHL-1). Confirmation of Integrity and Purity of Antibody

SDS-PAGE Analysis of Purified CD45RO MAb (UCHL-1). Confirmation of Integrity and Purity of Antibody

Immunofluorescence Analysis of human tonsil FFPE section stained with anti-CD45RO (UCHL-1) and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.

Immunofluorescence Analysis of human tonsil FFPE section stained with anti-CD45RO (UCHL-1) and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Chimpanzee, Common Marmoset, Human
Research Areas
Autophagy, Cancer, Developmental Biology, Immuno Oncology, Immunology, B Cell Markers, Dendritic Cell Marker, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane, Membrane raft
Gene Name
PTPRC
Positive Control
CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Alternate Names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Database Links

Entrez Gene ID
5788
SwissProt
P08575

Additional Information

Clone
UCHL-1
Chromosome Location
1q31.3
Mol. Weight of Antigen
180-185kDa

Related Antibodies

View All PTPRC Antibodies

Functions

  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
  • (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

Key References

  • Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138.3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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