Awaiting product image
Printable Datasheet

Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) [MLANA/7448R]

Clear

Summary

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Human
Isotype
IgG, kappa
Cellular Localization
Cytoplasm.
Gene Name
MLANA
Positive Control
COLO-38|SK-MEL-13 or SK-MEL-19 melanoma cell lines. Human skin or melanoma.
Immunogen
Recombinant fragment corresponding to the N-terminus of human Melan A protein
Alternate Names
Melanoma antigen recognized by T-cells 1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, MLANA

Database Links

Entrez Gene ID
2315
Unigene
154069
SwissProt
Q16655

Additional Information

Clone
MLANA/7448R
Human Gene Symbol
MLANA
Chromosome Location
9p24.1
Mol. Weight of Antigen
20-22kDa (doublet)

Functions

  • Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&deg
  • C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25.
  • Marincola FM, et. al. Journal of Immunotherapy with Emphasis on Tumor Immunology, 1996, 19(3):192-205.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “MART-1 / Melan-A / MLANA (Melanoma Marker)”

Your email address will not be published. Required fields are marked *

Related Antibodies

View All MLANA Antibodies

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies

Company Info
Antibodies
View All
Hosts
View All
Isotype
View All
Resources
Newsletter Signup NeoBiotechnologies
Updates & Sales

$0.00
510-376-5603