Complement 4d (C4d) (Acute Humoral Rejection Marker) [C4D204]



This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Product Properties & Targets

Species Reactivity
Cellular Localization
Intracytoplasmic vacuoles of endothelial cells and Secreted
Positive Control
Rejected Renal Transplant Tissue.
Recombinant human Complement 4d protein
Alternate Names
, Acidic Complement C4; Basic C4; C4-1; C4a Anaphylatoxin; C4A; C4A2; C4A3; C4A4; C4A6; C4b; C4B1; C4B12; C4d fragment; C4F; C4S; Complement C4 Gamma Chain; Complement Component 4A; Complement Component 4B

Database Links

Entrez Gene ID

Additional Information

Human Gene Symbol
Chromosome Location
Mol. Weight of Antigen
192kDa (predicted)

Known Applications & Suggested Dilutions

  • ELISA (For coating use Ab at 1-2ug/ml order Ab without BSA); Immunofluorescence (1:50-1:100);Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95&degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

  • Racusen LC et. al. Am J Transplant. 2003;3(6):708-14.
  • Collins AB et. al. J Am Soc Nephrol. 1999;10(10):2208-14.
  • Sacks SH et. al. Curr Opin Nephrol Hypertens. 2002;11(6):627-8.

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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