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CD71 / Transferrin Receptor (TFRC) [66IG10]

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Summary

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Product Properties & Targets

Host
Mouse
Species Reactivity
Human
Research Areas
Cancer, Cardiovascular
Isotype
IgG1, kappa
Cellular Localization
Cell surface
Gene Name
TFRC
Positive Control
CCRF-CEM|Jurkat or K-562. Human placenta or breast carcinoma.
Immunogen
Human thymocytes (T-cells)
Alternate Names
Transferrin receptor protein 1 | Transferrin receptor protein 1, serum form, T9, p90, Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Database Links

Entrez Gene ID
7037
Unigene
529618
SwissProt
P02786

Additional Information

Clone
66IG10
Human Gene Symbol
TFRC
Chromosome Location
3q29
Mol. Weight of Antigen
85-95kDa (monomer); 190kDa (dimer)

Functions

  • Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).
  • (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Known Applications & Suggested Dilutions

  • Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100);Optimal dilution for a specific application should be determined.

Key References

  • K. Moolenaar et al. Cancer research 50,1102-1106, 1990.
  • Van de Rijna M, Geurts van Kessel AHM, Kroezen V, van Agthoven AJ, Verstijnen K, Terhorst C, Hilgers J: Cytogenet Cell Genet 1983;36:525-531.
  • Oudermans et al. Cancer, 1986; 58:1252.

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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