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Home >> Antibodies >> PAX8 (Renal Cell Marker)
PAX8 (Renal Cell Marker)

Mouse Monoclonal Antibody [Clone PAX8/1492]

Catalog #
Size
20 ug
100 ug
100 ug
Price (USD)
199.00
429.00
429.00
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Catalog No
Size
20 ug
100 ug
100 ug
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Price (USD)
199.00
429.00
429.00
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Western Blot Analysis (A) Recombinant Protein (B) Raji cell lysate Using PAX8 Mouse Monoclonal Antibody (PAX8/1492).

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with PAX8 Mouse Monoclonal Antibody (PAX8/1492).

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Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with PAX8 Mouse Monoclonal Antibody (PAX8/1492).

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Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with PAX8 Mouse Monoclonal Antibody (PAX8/1492).

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SDS-PAGE Analysis Purified PAX8 Mouse Monoclonal Antibody (PAX8/1492). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing >19,000 full-length human proteins using PAX8 Mouse Monoclonal Antibody (PAX8/1492)

Product Details

Synonyms

Paired box 8; Paired box gene 8; paired box homeotic gene 8; Paired box protein Pax-8; Paired domain gene 8; PAX8

Positive Control

Raji cells. Renal Cell Carcinoma (RCC).

Known Applications & Suggested Dilutions

Flow Cyt, IF, WB, IHC-P         More Details
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human PAX8 protein

Species Reactivity

Human. Others not known.

Host / Ig Isotype

Mouse / IgG2a, kappa

Specificity & Comments

Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Related Products


Western Blot Analysis A) Recombinant Protein (B) Raji cell lysate Using PAX8 Mouse Monoclonal Antibody (PAX8/1491).
PAX8 (Renal Cell Marker)
[clone PAX8/1491]
Applications

WB, IHC

Reactivity

Human

Formalin-fixed, paraffin-embedded human Kidney stained with Adiponectin Mouse Monoclonal Antibody (ADPN/1370).
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[clone ADPN/1370]
Applications

WB, IHC

Reactivity

Human

Formalin-fixed, paraffin-embedded human Tonsil stained with AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/1909).
AIF1 / Iba1 (Microglia Marker)
[clone AIF1/1909]
Applications

WB, IHC

Reactivity

Human

Western Blot Analysis of HepG2 cell lysate using Albumin Mouse Monoclonal Antibody (ALB/2356).
Albumin (Transport Protein)
[clone ALB/2356]
Applications

WB, IHC

Reactivity

Human


200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tong GX,Yu WM,Beaubier NT,Weeden EM,Hamele-Bena D,Mansukhani MM,O'Toole KM. Expression of PAX8 in normal and neoplastic renal tissues: an immunohistochemical study. Mod Pathol.2009 Sep;22(9):1218-27.

Bioinformatics

  •  Entrez Gene ID: 7849
  •  Gene Symbol: PAX8
  •  SwissProt: Q06710
  •  Chromosome Location: 2q13
  •  Unigene: 469728
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