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Home >> Antibodies >> GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker)
GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker)

Mouse Monoclonal Antibody [Clone PIP/1571]

Catalog #
Size
20 ug
100 ug
100 ug
Price (USD)
199.00
429.00
429.00
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Catalog No
Size
20 ug
100 ug
100 ug
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Price (USD)
199.00
429.00
429.00
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Western Blot Analysis (A) Human Pancreas (B) HepG2 cell lysate Using GCDFP-15 Mouse Monoclonal Antibody (PIP/1571).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GCDFP-15 Mouse Monoclonal Antibody (PIP/1571).

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SDS-PAGE Analysis Purified GCDFP-15 Mouse Monoclonal Antibody (PIP/1571). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using GCDFP-15 (PIP) Mouse Monoclonal Antibody (PIP/1571)

Product Details

Synonyms

BRST-2; GCDFP-15; gp17; GPIP4; Gross cystic disease fluid protein 15; Prolactin-induced protein (PIP); Prolactin-inducible protein (PIP); Secretory actin-binding protein (SABP)

Positive Control

HepG2 cells. Breast or Pancreas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, WB, IHC-P         More Details
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human GCDFP-15 protein fragment (around aa 41-146) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic (Golgi bodies) and Secreted

Species Reactivity

Human. Others-not known.

Host / Ig Isotype

Mouse / IgG2a, kappa

Specificity & Comments

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wick MR., et al. Hum Pathol. 1989 Mar;20(3):281-7.

Bioinformatics

  •  Entrez Gene ID: 5304
  •  Gene Symbol: PIP
  •  SwissProt: P12273
  •  Chromosome Location: 7q34
  •  Unigene: 99949
DOC#01571