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Home >> Antibodies >> Calnexin (Endoplasmic Reticulum Marker)
Calnexin (Endoplasmic Reticulum Marker)

Mouse Monoclonal Antibody [Clone CANX/1541]

Catalog #
Size
20 ug
100 ug
100 ug
Price (USD)
199.00
429.00
429.00
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Catalog No
Size
20 ug
100 ug
100 ug
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Price (USD)
199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Small Intestine Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).

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Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).

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SDS-PAGE Analysis Purified Calnexin Mouse Monoclonal Antibody (CANX/1541).nConfirmation of Purity and Integrity of Antibody.

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Confocal immunofluorescence image of HeLa cells using Purified Calnexin Mouse Monoclonal Antibody (CANX/1541) labeled in Green. Reddot is used to label the nuclei Red.

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Western Blot Analysis of Human Kidney lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).

Product Details

Synonyms

Calnexin; CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; P90

Positive Control

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Known Applications & Suggested Dilutions

ELISA, WB, IHC-P         More Details
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blotting (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Others not known.
Does not react with Rat

Host / Ig Isotype

Mouse / IgG2b, kappa

Specificity & Comments

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Williams, D.B. 1995. Calnexin: a molecular chaperone with a taste for carbohydrate. Biochem. Cell Biol. 73: 123-132.

Bioinformatics

  •  Entrez Gene ID: 821
  •  Gene Symbol: CANX
  •  SwissProt: P27824
  •  Chromosome Location: 5q35.3
  •  Unigene: 567968
DOC#01541