Formalin-fixed, paraffin-embedded human Tonsil stained with ZAP70 Mouse Monoclonal Antibody (2F3.2)
SDS-PAGE Analysis Purified ZAP70 Mouse Monoclonal Antibody (2F3.2). Confirmation of Purity and Integrity of Antibody
Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Mouse Monoclonal Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).
Flow Cytometric Analysis of PFA-fixed Jurkat cells. ZAP70 Mouse Monoclonal Antibody (2F3.2) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive Purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.