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Home >> Antibodies >> Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)

Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)

Rabbit Polyclonal Antibody

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Mesothelioma stained with Wilm's Tumor Rabbit Polyclonal Antibody Antibody.

Product Details

Synonyms

WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1

Positive Control

K562 cells. Wilm's Tumor, mesothelioma or fetal kidney.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human WT1 protein

Cellular Localization

Nuclear and Cytoplasmic

Species Reactivity

Human. Rat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

47-55kDa

Specificity & Comments

Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

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200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Rauscher FJ. The WT1 Wilms tumor gene product: A developmentally regulated transcription factor in the kidney that functions as a tumor suppressor. FASEB J 1993; 7:896

Bioinformatics

  •  Entrez Gene ID: 7490
  •  SwissProt: P19544
  •  Chromosome Location: 11p13
  •  Unigene: 591980
NBT01665