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Home >> Antibodies >> Ubiquitin (Autophagy Marker)

Ubiquitin (Autophagy Marker)

Mouse Monoclonal Antibody [Clone UBB/2122]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin Mouse Monoclonal Antibody (UBB/2122).

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Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin Mouse Monoclonal Antibody (UBB/2122).

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SDS-PAGE Analysis Purified Ubiquitin Mouse Monoclonal Antibody (UBB/2122). Confirmation of purity and integrity.

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Western Blot Analysis of human Raji cell lysate using Ubiquitin Mouse Monoclonal Antibody (UBB/2122).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Ubiquitin Mouse Monoclonal Antibody (UBB/2122)

Product Details

Synonyms

Epididymis secretory protein Li 50; Polyubiquitin B; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; Ubiquitin; Ubiquitin B

Positive Control

HeLa or Jurkat cells. Alzheimer's Brain.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Cellular Localization

Cell Surface, Cytoplasmic and Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

9kDa

Specificity & Comments

Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hochstrasser, M. 1995. Ubiquitin, proteasomes and the regulation of intracellular protein degradation. Curr. Opin. Cell Biol. 7: 215-223.

Bioinformatics

NBT02122