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Home >> Antibodies >> TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker)

TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rNX2.1/690]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with TTF-1 Mouse Recombinant Monoclonal Antibody (rNX2.1/690).

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Formalin-fixed, paraffin-embedded human Thyroid stained with TTF-1 Mouse Recombinant Monoclonal Antibody (rNX2.1/690).

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SDS-PAGE Analysis Purified TTF-1 Mouse Recombinant Monoclonal Antibody (rNX2.1/690). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

BCH, Benign chorea, BHC, Homeobox protein Nkx2.1, NK2 homeobox 1, NKX2.1, NKX2A, TEBP, Thyroid nuclear factor-1, Thyroid specific enhancer binding protein, Thyroid transcription factor-1 (TTF-1), Tin man, TITF1, TTF-1

Positive Control

MAD109, MLE-15, H441-4 or H345 cells. Normal thyroid or lung.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human TTF-1 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40kDa

Specificity & Comments

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Related Products



200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wert, S.E., et al. 2002. Increased expression of TTF-1 in respiratory epithelial cells inhibits alveolarization and causes pulmonary inflammation. Dev. Biol. 242: 75-87.

Bioinformatics

  •  Entrez Gene ID: 7080
  •  Gene Symbol: NKX2-1
  •  SwissProt: P43699
  •  Chromosome Location: 14q13.3
  •  Unigene: 94367
NBT01853