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Home >> Antibodies >> TNF-alpha(TumorNecrosis Factor alpha)

TNF-alpha(TumorNecrosis Factor alpha)

Mouse Monoclonal Antibody [Clone TNF706]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Erdheim–Chester disease (polyostoticsclerosing histiocytosis) stained with Tumor Necrosis Factor alpha Mouse Monoclonal Antibody (TNF706).

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Immunofluorescence staining of PFA-fixed HePG2 cells using Tumor Necrosis Factor (TNF alpha) Mouse Monoclonal Antibody (TNF706) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.

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Formalin-fixed, paraffin-embedded rat pancreas stained with TNF alpha Mouse Monoclonal Antibody (TNF706).

Product Details

Synonyms

APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor

Positive Control

HePG2, HeLa, HL-60, or A431 cells. Macrophages in lymph node or tonsil (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC         More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-paraffin) (2-4ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant N-terminal fragment of human TNF alpha

Cellular Localization

Cytoplasm. Extracellular (secreted).

Species Reactivity

Human, Mouse, Rat, Rabbit, Cat, Dog and Zebrafish.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Related Products



200ug/ml of Ab purified by Protein L. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.

Key References

  1. McLaughlin PJ; Elwood NJ; Ramadi LT, Pica MR, McKenzie IF. Improvement in sensitivity of enzyme-linked immunosorbent assay for tumor necrosis factor. Immunol Cell Biol, 1990, 68:51-5.

Bioinformatics

  •  Entrez Gene ID: 7124
  •  Gene Symbol: TNF
  •  SwissProt: P01375
  •  Chromosome Location: 6p21.33
  •  Unigene: 241570