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Home >> Antibodies >> TLE1 (Synovial Sarcoma Marker)

TLE1 (Synovial Sarcoma Marker)

Mouse Monoclonal Antibody [Clone TLE1/2051]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with TLE1 Mouse Monoclonal Antibody (TLE1/2051).

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SDS-PAGE Analysis Purified TLE1 Mouse Monoclonal Antibody (TLE1/2051). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of human HeLa cell lysate using TLE1 Mouse Monoclonal Antibody (TLE1/2051).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using TLE1 Mouse Monoclonal Antibody (TLE1/2051).

Product Details

Synonyms

Enhancer of split groucho 1 (ESG1); Enhancer of split groucho-like protein 1; GRG1; TLE1; Transducin like enhancer of split 1; Transducin-like enhancer protein 1

Positive Control

HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

83kDa

Specificity & Comments

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that isencoded by theTLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Jagdis A, et al. Prospective evaluation of TLE1 as a diagnostic immunohistochemical marker in synovial sarcoma. Am J Surg Pathol. 2009; 33:1743-51.

Bioinformatics

  •  Entrez Gene ID: 7088
  •  Gene Symbol: TLE1
  •  SwissProt: Q04724
  •  Chromosome Location: 9q21.32
  •  Unigene: 197320, 689805
NBT02051