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Home >> Antibodies >> Thyroglobulin (Thyroidal Cell Marker)

Thyroglobulin (Thyroidal Cell Marker)

Mouse Monoclonal Antibody [Clone 2H11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Thyroid stained with Thyroglobulin Monoclonal Antibody (2H11).

Product Details

Synonyms

AITD3, hTG, TDH3, Tg, Tgn

Positive Control

Thyroid.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml
30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human thyroid follicular cells

Cellular Localization

Cytoplasmic and secreted

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

660kDa (Dimeric Form)

Specificity & Comments

MAb 2H11 reacts with a partially defined epitope of human thyroglobulin. This epitope is different form the epitope recognized by MAb 6E1. Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Endocrinol. 127: 419430 (1990).
  2. Bellet, D, et al. J Clin Endocrin Metab 1983;56:530-533
  3. Ossendorp FA, et. al. Journal of Immunological Methods, 1989, 120(2):191-200.
  4. Moll. Cell. Endocrinol 66: 199205 (1989).

Bioinformatics

  •  Entrez Gene ID: 7038
  •  Gene Symbol: TG
  •  SwissProt: P01266
  •  Chromosome Location: 8q24.22
  •  Unigene: 654591
NBT00023