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Home >> Antibodies >> TGF-alpha (Transforming Growth Factor alpha)

TGF-alpha (Transforming Growth Factor alpha)

Mouse Monoclonal Antibody [Clone 1E8-G6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Monoclonal Antibody (1E8-G6)

Product Details

Synonyms

EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1

Positive Control

Jurkat or Ramos cells. Testicular Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.

Cellular Localization

Cytoplasmic and Extracellular (secreted)

Species Reactivity

Human. Rabbit. Zebrafish.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

~6kDa

Specificity & Comments

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bebok Z; Szekeres G; Horvath G; Duda E; Nemeth P. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use]. Orvosi Hetilap, 1993, 134(24):1303-7. Language: Hungarian.
  2. Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35.
  3. Nasim, M. M., Thomas, D. M., Alison, M. R., and Fil]ipe, M. I. Transforming growth factor a expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma - an immunohistochemical study, Histopathology. 20: 339-343, 1992.

Bioinformatics

  •  Entrez Gene ID: 7039
  •  Gene Symbol: TGFA
  •  SwissProt: P01135
  •  Chromosome Location: 2p13.3
  •  Unigene: 170009
NBT00785