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Home >> Antibodies >> SUMO-2/3

SUMO-2/3

Mouse Monoclonal Antibody [Clone SM23/496]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SUMO-2/3 Mouse Monoclonal Antibody (SM23/496)

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Western Blot of HeLa cell lysate using SUMO-2/3 Mouse Monoclonal Antibody (SM23/496)

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Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO-2/3 Mouse Monoclonal Antibody (SM23/496) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence staining of paraformaldehyde-fixed MCF-7 cells with SUMO-2/3 Mouse Monoclonal Antibody (SM23/496) followed by goat anti-Mouse IgG-CF488 (Green). Membrane are labeled with Phalloidin (Red).

Product Details

Synonyms

HSMT3; Sentrin 2; Small ubiquitin like modifier 2; Small ubiquitin like modifier protein 3; SMT3A; SMT3B; SMT3 homolog 1 (SMT3H1); SMT3 homolog 2 (SMT3H2); SMT3 homolog; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 2; SMT3 suppressor of mif two 3 homolog 3; Suppressor of mif two 3 homolog 2; Suppressor of mif two 3 homolog 3; Ubiquitin like protein SMT3A; Ubiquitin like protein SMT3B

Positive Control

MCF-7, HepG2, HeLa cells. Breast carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human SUMO2 protein

Cellular Localization

Nuclear (SUMO-2); Cytoplasmic (SUMO-3)

Species Reactivity

Human. Predicted to show a broad species reactivity.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

11-13kDa

Specificity & Comments

This MAb reacts with both SUMO-2 and SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bioinformatics

  •  Entrez Gene ID: 6613, 6612
  •  Gene Symbol: SUMO2 & SUMO3
  •  SwissProt: P61956, P55854
  •  Chromosome Location: 17q25.1 (SUMO-2) & 21q22.3 (SUMO-3)
NBT00496